BLAST+ Basic Local Alignment Search Tool is a sequence homology search tool. Given a probe sequence (nucleotide or protein), BLAST compares it to a sequence database and picks out sequences with significant similarity to the probe sequence.
Below is a list of prerequisites for using BLAST+:
To load BLAST+ type the following:
$ module load gcc blast+
This will set the BLASTDB environment variable to /share/apps/blast-2.2.25+/db
If your databases are located elsewhere you will need to set the BLASTDB variable to point to that location.
A “.ncbirc” file under the home directory can be created to configure the BLASTDB variable.
Upon start, BLAST will read this file to get the path information it needs during BLAST searches.
Other blast commands